Genome-Wide Association Mapping of Freezing Tolerance Loci in Canola (Brassica napus L.)

Winter canola generally produces greater yields than spring canola. However, its range is limited due to inability withstand the harsh winter conditions that occur in many northern regions of U.S.A. To identify loci associated with freezing tolerance canola, we conducted a genome-wide association study (GWAS) using genotyped diversity panel containing 399 accessions consisting primarily One-month-old greenhouse grown plants were subsequently cold-acclimated for two months an environmental growth chamber prior phenotyping survival visual damage scale and chlorophyll fluorescence (Fv/Fo). There was reasonable correlation observed between ratings among top loci; results indicated some contributed both damage/tolerance photosynthetic efficiency. The resulting numerical values phenotypes used analyses identified SNPs. Thirteen significant markers on nine chromosomes scored, several showing significance multiple phenotypes. Twenty-five candidate genes as previously tolerance, photosynthesis, or cold-responsive Arabidopsis.